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. 2021 Apr 21;10(5):500. doi: 10.3390/pathogens10050500

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Kinetics and longevity of SSI trivalent and monovalent IPV neutralization antibodies. Female BALB/c mice (n = 9/group) received two intramuscular immunizations 3 weeks apart with indicated dose of trivalent (A) or monovalent (B–D) IPV vaccine alone (red) or formulated with Advax-CpG (blue) or CpG (green). Sera collected at indicated time-points were analyzed by virus neutralization assay against type1—Brunhilde, type 2—MEF-1 or type 3—Saukett virus. Serum neutralizing titers were calculated using the Kärber method (Mean ± SD). Dotted line placed at minimum protection level. SSI—Monovalent Type 1 at antigen dose of 0.1 and 1 DU not shown as they were below detection limit.