Figure 3.

Changes in systemic iron regulation at PD 15. (a) Liver hepcidin (Hamp) mRNA expression was assessed by real-time PCR (n = 7–8/group). Values with the mean ± SEM are plotted as fold change relative to CON means. (b) Relative expression of the iron exporter protein, ferroportin (Slc40a1), was assessed in the proximal small intestine (n = 4/group). Adjusted Slc40a1 band density was normalized to total protein with the Stain-Free™ method, and values are plotted relative to mean CON expression (%) as the means ± SEM. p-value summary: ****, p < 0.0001.