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. 2021 May 18;10(1):894–904. doi: 10.1080/22221751.2021.1925163

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Neutralization test based on SARS-CoV-2 pseudoviruses with miRFP reporter. (A,B) miRFP signals (top) and neutralization curves and NT₅₀ titers (bottom) to pseudoviruses Str and D614G at 72 h post-infection in HEK-293T-hACE2 cells of a convalescent-phase serum sample from a confirmed COVID-19 case (A) and neutralization curves and NT₅₀ titers of other 4 confirmed COVID-19 cases (B). Data are means and standard deviations of duplicates from one representative experiment of two. (C) Comparison between NT₅₀ titers to pseudoviruses Str and D614G for 15 confirmed COVID-19 cases. The two-tailed Wilcoxon rank signed test (GraphPad 6.0). (D) Relationship between NT₅₀ titers to pseudovirus Str and sampling days. (E-J) Relationship between NT₅₀ titers to pseudoviruses Str and PRNT₅₀ (E), PRNT₈₀ (F) or PRNT₉₀ (G) titers to SARS-CoV-2 USA-WA-1 strain (containing D614) and relationship between NT₅₀ titers to pseudoviruses D614G and PRNT₅₀ (H), PRNT₈₀ (I) or PRNT₉₀ (J) titers to SARS-CoV-2 USA-WA-1 strain. The two-tailed Spearman correlation test (GraphPad 6.0).