Figure 5.

Lappaol F (LAF) decreased the nuclear accumulation of YAP. (A) Cellular location of YAP (green color) in HeLa cells treated with LAF (50 µmol/L), VP (1 µmol/L) or EGF (20 ng/mL) alone or simultaneously for 48 h. YAP was detected by immunofluorescence staining, while the nuclei (blue color) were detected by staining with 4,6-diamino-2-phenyl indole. Pictures at the right side (scale bars: 10,000 nm) are an enlargement of yellow frames in the pictures at the left side (scale bars: 20,000 nm). (B) Viability of HeLa cells treated with LAF (0, 10, 25, 50 or 75 µmol/L) alone or simultaneously with VP (1 µmol/L) or EGF (20 ng/mL) for 48 h measured by sulforhodamine B assay. All data are expressed as the mean ± SD (n = 6). *p < 0.05, **p < 0.01, significantly different from the control group. DMSO: dimethyl sulfoxide; EGF: epidermal growth factor; VP: verteporfin; YAP: Yes-associated protein.