Figure 7.

Lappaol F (LAF) suppressed the growth of tumour xenografts in nude mice. SW480 colon cancer cells were subcutaneously injected into the left and right back regions of nude mice to induce tumours. Subsequently, the tumour-bearing mice were intravenously administrated with LAF (n = 8) or vehicle (n = 8) for 15 consecutive days, or paclitaxel every other day (n = 7, one mouse in this group died on the 8th day of administration). (A) Tumour volumes. (B) Tumour weight. (C) Body weight. (D) Tumours. (E) The apoptosis of tumours was determined using TUNEL (magnification, × 400). (F) Expression levels of proteins in tumour tissues were detected by western blotting. All data are expressed as the mean ± SD. *p < 0.05, **p < 0.01, significantly different from the vehicle group. Bcl-2: B cell lymphoma/leukemia-2; BIRC5: survivin/baculoviral IAP repeat containing 5; c-Myc: cellular myelocytomatosis oncogene; DAPI: 4,6-diamino-2-phenyl indole; TUNEL: terminal deoxynucleotidyl transferase–mediated dUTP nick end labelling; YAP: Yes-associated protein.