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Primer sets for amplification of PD-1, TIM-3, CTLA-4, and β-actin genes
| Gene Name | Sequences (5' -> 3') | Purification | References |
|---|---|---|---|
| β-actin-F | AGAGGGAAATCGTGCGTGAC | HPLC | (51) |
| β-actin-R | CAATAGTGATGACCTGGCCGT | HPLC | |
| PD-1-F | TGCTCAACAAGTATGTCAGAGG | HPLC | (52) |
| PD-1-R | ACACTAGGGACAGGTGCTGC | HPLC | |
| Tim-3-F | CCACGGAGAGAAATGGTTC | HPLC | (52) |
| Tim-3-R | CATCAGCCCATGTGGAAAT | HPLC | |
| CTLA4-F | GCT TCC TAG ATT ACC CCT TCT GC | HPLC | (53) |
| CTLA4-R | CGG GCA TGG TTC TGG ATCA | HPLC |
