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. 2021 May 21;59(1):47. doi: 10.3892/ijo.2021.5227

Figure 3.

Figure 3

miR-153-3p inhibits the invasion, migration and EMT process in RB cells in vitro. (A) Transwell assay was used to determine the invasion of RB cells following transfection of miR-153 mimics into WERI-RB-1 and Y79 cells. The results (mean ± SD) are from three independent experiments (**P<0.01 vs. mimics NC). (B) Wound healing assay was used to determine the migration of RB cells following transfection of miR-153 mimics into WERI-RB-1 and Y79 cells. The results (mean ± SD) are from three independent experiments (**P<0.01 vs. mimics NC). (C) Western blot analysis was used to determine the expression level of EMT-associated proteins following transfection of miR-153 mimics into WERI-RB-1 and Y79 cells. (D) The expression levels of Snail, Twist and ZEB1 were measured by western blot analysis following transfection of miR-153 mimics into WERI-RB-1 and Y79 cells. The results (mean ± SD) are from three independent experiments (**P<0.01 vs. mimics NC). RB, retinoblastoma; EMT, epithelial-mesenchymal transition; ZEB1, zinc finger E-box binding homeobox 1.