Skip to main content
. 2021 May 21;59(1):47. doi: 10.3892/ijo.2021.5227

Figure 4.

Figure 4

IGF1R is a target of miR-153 and is upregulated in RB tissues and cells. (A) Prediction of IGF1R as a target of miR-153-3p. (B) The transfection efficiency of miR-153 inhibitor was assessed by RT-qPCR. **P<0.01 vs. control. (C and D) Firefly luciferase reporter activity in Y79 cells and WERI-RB-1 cells transfected with mimics NC or miR-153 mimics or inhibitor NC or miR-153 inhibitor following transfection with wt-IGF1R-3′-UTR reporter or mut-IGF1R-3′-UTR reporter plasmid, respectively (**P<0.01 vs. mimics NC, ##P<0.01 vs. inhibitor NC). (E and F) mRNA level of IGF1R in Y79 cells and WERI-RB-1 cells transfected with miR-153 inhibitor and miR-153 mimics measured by RT-qPCR (**P<0.01 vs. mimics NC, ##P<0.01 vs. inhibitor NC). (G) The relative expression levels of IGF1R in two paired RB tissues and normal retinal tissues were measured by immunohistochemistry (**P<0.01 vs. ARPE-19). (H) Relative expression of IGF1R in RB cells compared to ARPE-19 cells. (I) Relative expression of IGF1R in RB tissues compared to normal retinal tissues. (J) Correlation between IGF1R and miR-153 expression analyzed using Pearson's correlation coefficient in RB tissues. RB, retinoblastoma; IGF1R, insulin-like growth factor 1 receptor.