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. 2021 May 21;59(1):47. doi: 10.3892/ijo.2021.5227

Figure 6.

Figure 6

Overexpression of IGF1R impairs the effects of miR-153 on RB cells. (A) Protein expression of IGF1R was examined by western blot analysis in WERI-RB-1 and Y79 cells following pcDNA-IGF1R transfection. The results (mean ± SD) are from three independent experiments (**P<0.01 vs. pcDNA vector group). (B) Expression level of IGF1R in RB cells determined by western blot analysis. WERI-RB-1 and Y79 cells were co-transfected with miR-153 mimics, mimics NC, or pcDNA-IGF1R. The results (mean ± SD) are from three independent experiments (**P<0.01 vs. mimics NC group; ##P<0.01 vs. miR-153 mimics group). (C) Cell viability was measured in WERI-RB-1 cells transfected with mimics NC or miR-153 mimics or miR-153 mimics plus pcDNA-IGF1R. The results (mean ± SD) are from three independent experiments (*P<0.05 and **P<0.01 vs. miR-153 mimics group). (D) Cell viability was measured in Y79 cells transfected with mimics NC or miR-153 mimics or miR-153 mimics plus pCDNA-IGF1R. The results (mean ± SD) are from three independent experiments (*P<0.05 and **P<0.01 vs. miR-153 mimics group). (E) Apoptosis of WERI-RB-1 and Y79 cells transfected with mimics NC or miR-153 mimics or miR-153 mimics plus pCDNA-IGF1R, was measured by flow cytometric analysis. The results (mean ± SD) are from three independent experiments (*P<0.05 and **P<0.01 vs. mimics NC group; ##P<0.01 vs. miR-153 mimics group). (F) Invasion of WERI-RB-1 and Y79 cells transfected with mimics NC or miR-153 mimics or miR-153 mimics plus pCDNA-IGF1R, was measured by Transwell assay. The results (mean ± SD) are from three independent experiments (*P<0.05 and **P<0.01 vs. mimics NC group; #P<0.05 and ##P<0.01 vs. miR-153 mimics group). (G) Migration of WERI-RB-1 and Y79 cells transfected with mimics NC or miR-153 mimics or miR-153 mimics plus pCDNA-IGF1R, was measured by wound healing assay. The results (mean ± SD) are from three independent experiments (*P<0.05 and **P<0.01 vs. mimics NC group; ##P<0.01 vs. miR-153 mimics group). RB, retinoblastoma; IGF1R, insulin-like growth factor 1 receptor.