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. Author manuscript; available in PMC: 2021 May 24.
Published in final edited form as: Cell Syst. 2020 Mar 18;10(3):223–239.e9. doi: 10.1016/j.cels.2020.02.008

Fig.7. SCFA promote production of IL-13 and other inflammatory cytokines exclusively in activated T cells under static conditions.

Fig.7

(A) Network of significantly enriched pathways based on KEGG in Treg/Th17 cells stimulated with IL-12 and IFN-γ (B) Venn diagram showing shared and unique significantly downregulated (left) or upregulated (right) genes among IL-12/IFN-γ stimulated Treg/Th17 cells +/- 1mM of total SCFA (magenta), unstimulated Treg/Th17 cells +/- 1mM of total SCFA (grey), IL-12/IFN-γ stimulated Treg/Th17 cells with SCFA over unstimulated T cells with SCFA (cyan), IL-12/IFN-γ stimulated Treg/Th17 cells over unstimulated T cells (orange). (C,D) Representative networks of downregulated pathways (C) or upregulated pathways (D) enriched based on REACTOME exclusively in IL-12/IFN-γ stimulated Treg/Th17 cells treated with 1mM of total SCFA. RNAseq analysis was performed on pooled cells from 3 independent experiments. (E) Multiplexed cytokines/chemokines in supernatants collected from each stimulated or unstimulated group with or without SCFA after 48 h of incubation. *FDR<0.05; **FDR<0.01; ***FDR<0.001; ****FDR<0.0001.