| Gene (Saccharomyces cerevisiae) |
Nyv1 |
Saccharomyces Genome Database |
SGD:S000004083 |
|
| Gene (Saccharomyces cerevisiae) |
Vam3 |
Saccharomyces Genome Database |
SGD:S000005632 |
|
| Gene (Saccharomyces cerevisiae) |
Vti1 |
Saccharomyces Genome Database |
SGD:S000004810 |
|
| Gene (Saccharomyces cerevisiae) |
Vam7 |
Saccharomyces Genome Database |
SGD:S000003180 |
|
| Gene (Saccharomyces cerevisiae) |
Ypt7 |
Saccharomyces Genome Database |
SGD:S000004460 |
|
| Gene (Saccharomyces cerevisiae) |
Sec17 |
Saccharomyces Genome Database |
SGD:S000000146 |
|
| Gene (Saccharomyces cerevisiae) |
Sec18 |
Saccharomyces Genome Database |
SGD:S000000284 |
|
| Gene (Saccharomyces cerevisiae) |
Vps33 |
Saccharomyces Genome Database |
SGD:S000004388 |
|
| Gene (Saccharomyces cerevisiae) |
Vps39 |
Saccharomyces Genome Database |
SGD:S000002235 |
|
| Gene (Saccharomyces cerevisiae) |
Vps41 |
Saccharomyces Genome Database |
SGD:S000002487 |
|
| Gene (Saccharomyces cerevisiae) |
Vps16 |
Saccharomyces Genome Database |
SGD:S000005966 |
|
| Gene (Saccharomyces cerevisiae) |
Vps11 |
Saccharomyces Genome Database |
SGD:S000004844 |
|
| Gene (Saccharomyces cerevisiae) |
Vps18 |
Saccharomyces Genome Database |
SGD:S000004138 |
|
| Peptide, recombinant protein |
GST-R (Nyv1) |
PMID:18650938
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Qa (Vam3) |
PMID:18650938
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Qb (Vti1) |
PMID:18650938
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
His-R (Nyv1) |
PMID:22174414
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-sQa (soluble) |
PMID:28637767
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
MBP-sQb (soluble) |
PMID:24088569
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Qb (Vti1)3Δ |
This study |
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Qa (Vam3)3Δ |
This study |
|
Purified from E. coli. |
| Peptide, recombinant protein |
Qc (Vam7) |
PMID:17699614
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Qc (Vam7)-tm |
PMID:23071309
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Qc (Vam7) C208S, M250C |
This study |
|
Purified from E. coli. |
| Peptide, recombinant protein |
Qc (Vam7) C208S, A316C |
This study |
|
Purified from E. coli. |
| Peptide, recombinant protein |
MBP-Cys-sQb (SNARE domain) |
PMID:28637767
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
MBP-Cys-sQb (SNARE domain) |
PMID:28637767
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Ypt7 |
PMID:24088569
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Ypt7-TM |
PMID:31235584
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Sec17 |
PMID:19414611
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Sec17 FSMS |
PMID:28925353
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Sec17 KEKE |
PMID:28925353
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
Sec17 LALA |
PMID:19414611
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Sec17 6AtoN |
This study |
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Sec17-TM |
PMID:28718762
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
GST-Sec17-TM FSMS |
PMID:28718762
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
TEV protease |
PMID:18007597
|
|
Purified from E. coli. |
| Peptide, recombinant protein |
HOPS |
PMID:18385512
|
|
Purified from Saccharomyces cerevisiae. |
| Antibody |
Anti-Vam3 (rabbit polyclonal) |
PMID:12566429
|
Wickner lab stock |
WB: 1:2000 |
| Antibody |
Anti-Nyv1 (rabbit polyclonal) |
PMID:10385523
|
Wickner lab stock |
WB: 0.65 μg/ml |
| Antibody |
Anti-Vti1 (rabbit polyclonal) |
PMID:18007597
|
Wickner lab stock |
WB: 0.47 μg/ml |
| Antibody |
Anti-Vam7 (rabbit polyclonal) |
PMID:14734531
|
Wickner lab stock |
WB: 0.1 μg/ml |
| Antibody |
Anti-Vps16 (rabbit polyclonal) |
PMID:18007597
|
Wickner lab stock |
WB: 1 μg/ml |
| Antibody |
Anti-Vps33 (rabbit polyclonal) |
PMID:10944212
|
Wickner lab stock |
Inhibition assay: 1 μg |
| Antibody |
Anti-Sec18 (rabbit polyclonal) |
PMID:11483507
|
Wickner lab stock |
Inhibition assay: 1 μg |
| Chemical compound, drug |
Cy5-derivatized streptavidin |
SeraCare Life Sciences |
5270–0023 |
|
| Chemical compound, drug |
Biotinylated PhycoE |
Thermo Fisher Scientific |
p811 |
|
| Chemical compound, drug |
Streptavidin |
Thermo Fisher Scientific |
434302 |
|
| Chemical compound, drug |
1,2-dilinoleoyl-sn-glycero-3-phosphocholine |
Avanti polar lipids |
850385 |
|
| Chemical compound, drug |
1,2-dilinoleoyl-sn-glycero-3-phospho-L-serine |
Avanti polar lipids |
840040 |
|
| Chemical compound, drug |
1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamine |
Avanti polar lipids |
850755 |
|
| Chemical compound, drug |
1,2-dilinoleoyl-sn-glycero-3-phosphate |
Avanti polar lipids |
840885 |
|
| Chemical compound, drug |
L-α-phosphatidylinositol |
Avanti polar lipids |
840044 |
|
| Chemical compound, drug |
1,2-dipalmitoyl-sn-glycerol |
Avanti polar lipids |
800816 |
|
| Chemical compound, drug |
Ergosterol |
Sigma |
45480 |
|
| Chemical compound, drug |
PI(3)P |
Echelon Bioscience |
P-3016 |
|
| Chemical compound, drug |
Rhodamine DHPE |
Invitrogen |
L1392 |
|
| Chemical compound, drug |
NBD-PE |
Invitrogen |
N360 |
|
| Chemical compound,drug |
Marina-blue |
Invitrogen |
M12652 |
|
| Software and algorithms |
UN-SCAN-IT |
Silk Scientific |
|
|
| Chemical compound, drug |
Alexa Fluor 568 C5-maleimide |
Thermo Fisher Scientific |
A20341 |
|
| Chemical compound, drug |
Oregon Green 488 Maleimide |
Thermo Fisher Scientific |
O6034 |
|
| Chemical compound, drug |
Oregon Green 488 Maleimide |
Thermo Fisher Scientific |
O6034 |
|
| Chemical compound, drug |
Pierce TCEP-HCl |
Thermo Fisher Scientific |
20490 |
|
| Chemical compound, drug |
L-cysteine |
Sigma-Aldrich |
30089 |
|
