Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-11268 | Female |
| Cell line (Homo sapiens) | HeLa | ATCC | CCL-2 | Female |
| Cell line (Homo sapiens) | HT-29 | ATCC | HTB-38 | Female |
| Cell line (Homo sapiens) | MCF7 | ATCC | HTB-22 | Female |
| Cell line (Homo sapiens) | KGN | Nishi et al., 2001 | N/A | Female |
| Cell line (Homo sapiens) | L929(Ripk3-/-) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | KGN/TO-RIPK3 | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | KGN/TO- RIPK3(S164A/T165A) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | MCF7/TO-RIPK3 | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | MCF7/TO- RIPK3(D160N) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | MCF7/TO- RIPK3(K50A) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | MCF7/TO- RIPK3(S164A/T165A) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Cell line (Homo sapiens) | MCF7/TO-RIPK3 (RIPK1-/-/Caspase-8-/-/FADD-/-/cFLIP-/-) | Dr. Xiaodong Wang lab at National Institute of Biological Sciences, Beijing | N/A | |
| Antibody | Anti-RIPK3 (Rabbit polyclonal) |
ProSci | Cat# 2283; RRID:AB_203256 | WB (1:1000) |
| Antibody | Anti-p-S164/T165-RIPK3 (Rabbit monoclonal) |
Abcam | Cat# Ab255705; | Firstly described in this paper; WB (1:1000) IF (1:100) |
| Antibody | Anti-RIPK3 (Mouse monoclonal) |
LSBio | Cat# LS-C336804 | WB (1:1000) |
| Antibody | Anti-GAPDH-HRP (Mouse monoclonal) |
MBL | Cat# M171-1; RRID:AB_10699462 | WB (1:20,000) |
| Antibody | Anti-β-actin-HRP (Rabbit polyclonal) |
MBL | Cat# PM053-7; RRID:AB_10697035 | WB (1:20,000) |
| Antibody | Anti-Tubulin-HRP (Rabbit polyclonal) |
MBL | Cat# PM054-7; RRID:AB_10695326 |
WB (1:20,000) |
| Antibody | Anti-Flag-HRP (Mouse monoclonal) |
Sigma-Aldrich | Cat# A8592; RRID:AB_439702 | WB (1:10,000) |
| Antibody | Anti-RIPK1 (Rabbit polyclonal) |
Cell Signaling | Cat# 3493S; RRID:AB_2305314 | WB (1:1000) |
| Antibody | Anti-cleaved-caspase3 (Rabbit polyclonal) |
Cell Signaling | Cat# 9661; RRID:AB_2341188 |
WB (1:1000) IF(1:100) |
| Antibody | Anti-Mouse-MLKL (Rabbit polyclonal) |
ABGENT | Cat# AP14272b; RRID:AB_11134649 | WB (1:1000) |
| Antibody | Anti-Human-MLKL (Rabbit monoclonal) |
Abcam | Cat# ab184718; RRID:AB_2755030 |
WB (1:1000) |
| Antibody | Anti-caspase-8 (Mouse monoclonal) |
Cell Signaling | Cat# 9746; RRID:AB_2275120 |
WB (1:1000) |
| Antibody | anti-FADD (Rabbit polyclonal) |
Cell Signaling | Cat# 2782; RRID:AB_2100484 |
WB (1:1000) |
| Antibody | Anti-p-S227-RIP3 (Rabbit monoclonal) |
Abcam | Cat# ab209384; RRID:AB_2714035 | WB (1:1000) |
| Antibody | Anti-p-S232-RIP3 (Rabbit monoclonal) |
Abcam | Cat# ab222302 | WB (1:1000) |
| Antibody | Anti-cFLIP (Rabbit polyclonal) |
Abcam | Cat# ab6144; RRID:AB_305314 |
WB (1:1000) |
| Antibody | Anti-cleaved-caspase-3 (Mouse monoclonal) |
St John's Laboratory | Cat# STJ97448 | IHC (1:100) |
| Antibody | Anti-prostaglandin F2 alpha (PTGFR) (Rabbit polyclonal) |
Abcam | Cat# ab203342 | WB (1:1000) IF (1:100) |
| Antibody | Anti-HSP90 (Rabbit polyclonal) |
Proteintech | Cat# 13171-1-AP;RRID:AB_2120924 | WB (1:1000) IF (1:100) |
| Antibody | Anti-CDC37 (Rabbit monoclonal) |
Abcam | Cat# ab108305; RRID:AB_10861724 |
WB (1:1000) IF (1:100) |
| Antibody | anti-FLAG M2 (Mouse monoclonal) |
Sigma-Aldrich | Cat# F1840 | IHC (1:100) |
| Antibody | Anti-phospho-p44/42 MAPK (Rabbit monoclonal) |
Cell Signaling | Cat# 4370S; RRID:AB_2315112 |
WB (1:1000) |
| Antibody | Anti-p44/42 MAPK (Rabbit monoclonal) |
Cell Signaling | Cat# 4965S | WB (1:1000) |
| Antibody | Anti-phospho-MEK1/2 (Rabbit monoclonal) |
Cell Signaling | Cat# 9154S; RRID:AB_2138017 |
WB (1:1000) |
| Antibody | Anti-MEK1/2 (Rabbit monoclonal) |
Abcam | Cat# ab178876 | WB (1:1000) |
| Antibody | Donkey anti-Mouse, Alexa Fluor 488 (Mouse polyclonal) |
Thermo Fisher | Cat# A-21202; RRID:AB_141607 | IF (1:500) |
| Antibody | Donkey anti-Mouse, Alexa Fluor 555 (Mouse polyclonal) |
Thermo Fisher | Cat# A-31570; RRID:AB_2536180 | IF (1:500) |
| Antibody | Donkey anti-Rabbit, Alexa Fluor 488 (Rabbit polyclonal) |
Thermo Fisher | Cat# A-21206; RRID:AB_141708 | IF (1:500) |
| Antibody | Donkey anti-Rabbit, Alexa Fluor 555 (Rabbit polyclonal) |
Thermo Fisher | Cat# A-31572; RRID:AB_162543 |
IF (1:500) |
| Antibody | Anti-Flag M2 affinity gel | Sigma-Aldrich | A2220 | |
| Recombinant DNA reagent | pWPI-HA-3xFlag-RIPK3 | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(D160N) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(K50A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(AAAA) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag-mRIPK3 | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag-RIPK3(S164D/T165E) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(S164E) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(T165E) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(S164A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(T165A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- RIPK3(S164A/T165A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag-mRIPK3(D161N) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pWPI-HA-3xFlag- mRIPK3(S165D/T166E) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pLVX-Tight-HA-3xFlag-RIPK3 | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pLVX-Tight-HA-3xFlag- RIPK3(D160N) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pLVX-Tight-HA-3xFlag- RIPK3(K50A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pLVX-Tight-HA-3xFlag- RIPK3(S164A/T165A) | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pX458-GFP-RIPK1 | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pX458-GFP-caspase-8 | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pX458-GFP-FADD | This paper | N/A | Described in Materials and methods; available upon request |
| Recombinant DNA reagent | pX458-GFP-cFLIP | This paper | N/A | Described in Materials and methods; available upon request |
| Peptide, recombinant protein | 3xFlag peptide | ChinaPeptides | DYKDHDGDYKDHDIDYKDDDDK | 1 mg/ml |
| Software, algorithm | ImageJ | NIH | N/A | |
| Software, algorithm | Photoshop | Adobe | N/A | |
| Software, algorithm | Lasergene | DNASTAR | N/A | |
| Software, algorithm | GraphPad | GraphPad Software | N/A | |
| Software, algorithm | Prism | GraphPad Software | N/A | |
| Software, algorithm | Nikon A1-R | Nikon | https://www.nikoninstruments.com/Products/Confocal-Microscopes/A1R-HD |