Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) |
Male C57BL/6J mice | Charles River or Envigo | Stock#: 000664, RRID:IMSR_JAX:000664 |
|
| Genetic reagent (Mus musculus) |
Male heterozygousB6.SJL-Slc6a3tm1.1(cre)Bkmn/J mice | The Jackson Laboratory | Stock#: 006660, RRID:IMSR_JAX:006660 |
Maintained on C57BL/6J background |
| Genetic reagent (Mus musculus) |
Male heterozygousB6.Cg-Foxp2tm1.1(cre)Rpa/J mice | The Jackson Laboratory | Stock#: 030541, RRID:IMSR_JAX: 030541 |
Maintained on C57BL/6J background |
| Genetic reagent (Mus musculus) |
Male heterozygous ICR.Cg-Gad1tm1.1Tam/Rbrc mice |
Tamamaki et al., 2003. J Comp Neurol 467(1):60–79. doi:10.1002/cne.10905 | RRID:IMSR_RBRC03674 | Maintained on C57BL/6J background |
| Strain, strain background (adeno-associated virus) |
AAV2/1.EF1a.DIO.hChR2(H134R).eYFP | Addgene / U. Penn Vector Core |
Cat# 20298-AAV1, RRID:Addgene viral prep # 20298-AAV1 | |
| Strain, strain background (adeno-associated virus) |
AAV2/9.EF1a.DIO.hChR2 (H134R).eYFP |
Addgene / U. Penn Vector Core |
Cat# 20298-AAV9, RRID:Addgene viral prep # 20298-AAV9 | |
| Strain, strain background (adeno-associated virus) |
AAV2/1.EF1a.DIO.hChR2 (H134R).mCherry |
Addgene/Viral Vector Facility U. Zurich |
Cat# 20297-AAV1, RRID:Addgene viral prep # 20297-AAV1 | |
| Antibody | Anti-GFP (goat polyclonal) | GeneTex | Cat# GTX26673, RRID:AB_371426 | IF(1:500) |
| Antibody | Anti-GFP (rabbit polyclonal) | Invitrogen | Cat# A11122, RRID:AB_221569 | IF(1:750 or 1:1000) |
| Antibody | Anti-FoxP2 (rabbit polyclonal) | Abcam | Cat# AB16046, RRID:AB_2107107 | IF(1:1000) |
| Antibody | Anti-FoxP2 (mouse monoclonal) | Merck Millipore | Cat# MABE415, RRID:AB_2721039 | IF(1:1000) |
| Antibody | Anti-FoxP2 (sheep polyclonal) | R and D Systems | Cat# AF5647, RRID:AB_2107133 | IF(1:200), IHC(1:200) |
| Antibody | Anti-TH (rabbit polyclonal) | Millipore | Cat# AB152, RRID:AB_390204 | IF(1:1000), IHC (1:4000) |
| Antibody | Anti-TH (mouse monoclonal) | Merck Millipore |
Cat# MAB318, RRID:AB_2313764 | IF(1:1000) |
| Antibody | Anti-Bassoon (mouse monoclonal) | Abcam | Cat# AB82958, RRID:AB_1860018 | IF(1:500) |
| Antibody | Anti-VGAT (guinea pig polyclonal) | Synaptic Systems | Cat# 131 004, RRID:AB_887873 | IF(1:500) |
| Antibody | Anti-VGLUT1 (guinea pig polyclonal) | Chemicon/ Millipore |
Cat# AB5905, RRID:AB_2301751 | IF(1:3000) |
| Antibody | Anti-VGLUT2 (guinea pig monoclonal) | Chemicon/ Millipore |
Cat# AB5907, RRID:AB_2301731 | IF(1:5000) |
| Antibody | Anti-Goat Alexa 488 (donkey polyclonal) | Invitrogen/Life Technologies | Cat# A-11055, RRID:AB_2534102 | IF(1:1000) |
| Antibody | Anti-Rabbit Alexa 555 (donkey polyclonal) | Invitrogen/Life Technologies | Cat# A-31572, RRID:AB_162543 | IF(1:1000) |
| Antibody | Anti-Mouse Alexa 555 (goat polyclonal) | Invitrogen/Life Technologies | Cat# A-21424, RRID:AB_141780 | IF(1:1000) |
| Antibody | Anti-Rabbit Alexa 647 (donkey polyclonal) | Invitrogen/Life Technologies | Cat# A-31573, RRID:AB_2536183 | IF(1:1000) |
| Antibody | Anti-Mouse Alexa 633 (goat polyclonal) | Invitrogen/Life Technologies | Cat# A-21052, RRID:AB_2535719 | IF(1:1000) |
| Antibody | Anti-Rabbit Alexa 633 (goat polyclonal) | Invitrogen/Life Technologies | Cat# A-21071, RRID:AB_2535732 | IF(1:1000) |
| Antibody | Anti-Rabbit Alexa 488 (donkey polyclonal) | Life Technologies | Cat# A21206, RRID:AB_2535792 | IF(1:1000) |
| Antibody | Anti-Goat Cy5 (donkey polyclonal) | Jackson Immuno-research | Cat# 705-175-147, RRID:AB_2340415 | IF(1:400) |
| Antibody | Anti-Guinea pig DyLight 405 (donkey polyclonal) | Jackson Immuno-research | Cat# 706-475-148, RRID:AB_2340470 | IF(1:500) |
| Antibody | Anti-rabbit (goat polyclonal) | Vector Laboratories | Cat# BA-1000, RRID:AB_2313606 | IHC(1:500) |
| Antibody | Anti-goat/sheep (horse polyclonal) | Vector Laboratories | Cat# BA-9500, RRID:AB_2336123 | IHC(1:500) |
| Chemical compound, drug | Tetrodotoxin citrate (TTX) | Alomone Labs | Cat# T-550 | |
| Chemical compound, drug | 4-amino pyridine (4-AP) | Sigma Aldrich | Cat# 275875 | |
| Chemical compound, drug | Picrotoxin (PTX) | Sigma Aldrich | Cat# P1675 | |
| Chemical compound, drug | 6,7-dinitro quinoxaline-2,3-dione (DNQX) disodium salt | Tocris Bioscience | Cat# 2312 | |
| Chemical compound, drug | DL-2-Amino-5-phosphono pentanoic acid sodium salt (DL-AP5) | BioTrend | Cat# BN0086 | |
| Chemical compound, drug | Dopamine hydrochloride | Sigma Aldrich | Cat# H8502 | |
| Chemical compound, drug | Dihydrexidine hydrochloride (DH) | Tocris Bioscience | Cat# 0884 | |
| Chemical compound, drug | Quinpirole hydrochloride | Sigma Aldrich | Cat# Q102 | |
| Chemical compound, drug | CGP55845 hydrochloride | Tocris Bioscience | Cat# 1248 | |
| Chemical compound, drug | SCH23390 hydrochloride | Tocris Bioscience | Cat# 0925 | |
| Chemical compound, drug | Sulpiride | Tocris Bioscience | Cat# 0895 | |
| Chemical compound, drug | L-745870 trihydro- chloride | Tocris Bioscience | Cat# 1002 | |
| Software, algorithm | SPSS | IBM | RRID:SCR_002865 | |
| Software, algorithm | Igor Pro | WaveMetrics | RRID:SCR_000325 | |
| Software, algorithm | NeuroMatic | NeuroMatic | RRID:SCR_004186 | |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
| Software, algorithm | Fiji | Fiji | RRID:SCR_002285 | |
| Software, algorithm | pClamp 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | ZEN 2009 Software | Zeiss | RRID:SCR_013672 | |
| Software, algorithm | Huygens Suite 19.04 | Scientific Volume Imaging | RRID:SCR_014237 | |
| Software, algorithm | Imaris Software, versions 7.6.1 and 9.7.0 | Oxford Instruments, Bitplane | RRID:SCR_007370 | |
| Other | NeuroTrace stain 435/455 | Thermo Fisher Scientific | Cat# N21479 | |
| Other | Vectastain Elite ABC-Peroxidase kit | Vector Laboratories | Cat# PK-7100, RRID:AB_2336827 | |
| Other | CY3-Streptavidin | Dianova/ Jackson Immuno-Research |
Cat# 016-160-084, RRID:AB_2337244 | |
| Other | Pacific Blue-Streptavidin | Thermo Fisher Scientific | Cat# S11222 |