. Author manuscript; available in PMC: 2021 Dec 7.

Published in final edited form as: J Neurosci Res. 2020 Jun 7;100(1):35–47. doi: 10.1002/jnr.24640

Table 3.

Expression of the human Oprm1 splice variant mRNAs in the medial prefrontal cortex of saline control and heroin self-administration (SA) rats.

Rat OPRM1 splice variant category	Variants	ΔC(t) values^#
Rat OPRM1 splice variant category	Variants	Saline control	Heroin SA
7TM variants	rE1–2	7.48 ± 0.37	7.77 ± 0.25
	rMOR-1A	9.91 ± 0.28	12.43 ± 0.39^*** ↓
	rMOR-1B1	8.59 ± 0.17	9.71 ± 0.39^* ↓
	rMOR-1C1	15.27 ± 0.17	15.81 ± 0.15
	rMOR-1C2	15.31 ± 0.12	15.20 ± 0.34
	rMOR-1D	16.52 ± 0.40	15.97 ± 0.34
	rMOR-1H1	8.37 ± 0.31	8.63 ± 0.24
	rMOR-1H2	13.11 ± 0.33	13.12 ± 0.12
	rMOR-1i	13.21 ± 0.21	13.02 ± 0.48
6TM variants	rMOR-1G1	10.28 ± 0.36	8.76 ± 0.49^** ↑
6TM variants	rMOR-1G2	15.35 ± 0.45	16.05 ± 0.66
1TM variants	rMOR-1S	10.83 ± 0.34	11.12 ± 0.22
1TM variants	rMOR-1Z	17.10 ± 0.12	17.11 ± 0.17

^#:

ΔC(t) = C(t)_variant – C(t)_NF, where NF (Normalized Factor) = (C(t)_G3PDH x C(t)_18S)^1/2; ↑ & ↓: increased or decreased expression as compared to saline control rats, respectively; Two-Way ANOVA with uncorrected Fisher LSD showed a statistically significant effect among the variants, F(12, 129) = 194.3, p < 0.0001, between normal control subjects and heroin abusers, F(1, 129) = 4.034, p = 0.0467, and interaction, F(12, 129) = 4.005, p < 0.0001. Compared to Control subjects

^*:

p < 0.05

^**:

p <0.01

^***:

p < 0.001.