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. Author manuscript; available in PMC: 2021 May 24.
Published in final edited form as: Cell Rep. 2020 Jul 14;32(2):107881. doi: 10.1016/j.celrep.2020.107881

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER

Antibodies
Immunoblotting: Rabbit monoclonal anti-CD9 Abcam Cat# ab92726; RRID: AB_10561589
Immunoblotting: Rabbit polyclonal anti-Calnexin Abcam Cat# ab10286; RRID: AB_2069009
Immunoblotting: Mouse monoclonal anti-GM130 BD Biosciences Cat# 610823; RRID: AB_398142
Immunoblotting: Rabbit monoclonal anti-Flotillin-1 Cell Signaling Technology Cat# 18634; RRID: AB_2773040
Immunoblotting: Mouse monoclonal anti-Alix Santa Cruz Biotechnology Cat# sc-53540; RRID: AB_673819
Immunoblotting: Rat monoclonal anti-IL-4 Abcam Cat# ab11524; RRID: AB_298129
Immunoblotting: rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific Cat# 61–9520; RRID: AB_2533945
Immunoblotting: mouse IgG kappa binding protein (m-IgGκ BP)-HRP Santa Cruz Biotechnology Cat# sc-516102; RRID: AB_2687626
Immunoblotting: F(ab)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP Thermo Fisher Scientific Cat# A10547; RRID: AB_2534046
Flow Cytometry: Biotin anti-mouse/human CD45R/B220 antibody BioLegend Cat# 103204; RRID: AB_312989
Flow Cytometry: Biotin anti-mouse CD4 antibody Biolegend Cat# 100508; RRID: AB_312711
Flow Cytometry: Biotin anti-mouse CD8a antibody Biolegend Cat# 100704; RRID: AB_312743
Flow Cytometry: Brilliant Violet 421 anti-mouse CD16/32 antibody Biolegend Cat# 101332; RRID: AB_2650889
Flow Cytometry: Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) antibody Biolegend Cat# 108404; RRID: AB_313369
Flow Cytometry: PE/Cy7 anti-mouse CD150 (SLAM) antibody Biolegend Cat# 115914; RRID: AB_439797
Flow Cytometry: Biotin anti-mouse TER-119/Erythroid Cells antibody Biolegend Cat# 116204; RRID: AB_313705
Flow Cytometry: Brilliant Violet 510 anti-mouse CD41 antibody Biolegend Cat# 133923; RRID: AB_2564013
Flow Cytometry: Biotin anti-mouse CD127 (IL-7Rα) antibody Biolegend Cat# 135006; RRID: AB_2126118
Flow Cytometry: PE anti-mouse CD115 (CSF-1R) antibody Biolegend Cat# 135505; RRID: AB_1937254
Flow Cytometry: FITC anti-mouse Ly-6C antibody Biolegend Cat# 128006; RRID: AB_1186135
Flow Cytometry: PerCP/Cyanine5.5 anti-mouse/human CD11b antibody Biolegend Cat# 101228; RRID: AB_893232
Flow Cytometry: APC anti-mouse CD45 antibody Biolegend Cat# 103112; RRID: AB_312977
Flow Cytometry: c-Kit Monoclonal Antibody (2B8), APC-Cyanine7 Thermo Fisher Scientific Cat# A15423; RRID: AB_2534436
Flow Cytometry: CD34 Monoclonal Antibody (RAM34), FITC Thermo Fisher Scientific Cat# 11-0341-85; RRID: AB_465022
Flow Cytometry: Ly-6A/E (Sca-1) Monoclonal Antibody (D7), PE Thermo Fisher Scientific Cat# 12-5981-83; RRID: AB_466087
Flow Cytometry: CD48 Monoclonal Antibody (HM48-1), APC Thermo Fisher Scientific Cat# 17-0481-82; RRID: AB_469408
Flow Cytometry: CD135 (Flt3) Monoclonal Antibody (A2F10), PerCP-eFluor 710 Thermo Fisher Scientific Cat# 46-1351-82; RRID: AB_10733393
Flow Cytometry: APC Rat Anti-Mouse TNF (MP6-XT22) BD Biosciences Cat# 554420; RRID: AB_398553
Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 Thermo Fisher Scientific Cat# A-21208; RRID: AB_2535794
Anti-Mouse Macrophages / Monocytes Purified (Clone MOMA-2) (rat IgG2b) Cedarlane Cat# CL89154; RRID: AB_10086520
Rat anti-mouse CD206 Bio-Rad Laboratories Cat# MCA2235; RRID: AB_324622
TruStain FcX Antibody Biolegend Cat# 101320; RRID: AB_1574975
BV786 Streptavidin BD Biosciences Cat# 563858
CD14 MicroBeads, human Miltenyi Biotec Cat#130-118-906; RRID: AB_2665482
Anti-F4/80 MicroBeads, mouse Miltenyi Biotec Cat#130-110444
Anti-APC MicroBeads, mouse Miltenyi Biotec Cat# 130-090-855; RRID: AB_244367

Chemicals, Peptides and Recombinant Proteins
DiR (DiIC18(7) (1,1’-Dioctadecyl-3,3,3′,3′-
Tetramethylindotricarbocyanine Iodide))
Invitrogen Cat# D12731
Fast SYBR Green Master Mix Applied Biosystems Cat# 4385614
RBC Lysis Buffer (10X) BioLegend Cat# 420301
iScript Reverse Transcription Supermix Bio-Rad Laboratories Cat# 1708841
10% Mini-PROTEAN TGX Gels Bio-Rad Laboratories Cat# 4561034
4x Laemmli Sample Buffer Bio-Rad Laboratories Cat# 1610747
RIPA buffer (10x) Cell Signaling Technology Cat# 9806
Amersham ECL Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2232
Penicillin-Streptomycin GIBCO Cat# 15140122
L-Glutamine GIBCO Cat# 25030-081
Recombinant Murine IL-4 Peprotech Cat# 214-14
Recombinant Murine IL-4 Abcam Cat# ab9729
Recombinant Murine M-CSF Peprotech Cat# 315-02
Recombinant Human M-CSF Peprotech Cat# 300–25
Animal-Free Recombinant Murine TPO Peprotech Cat# AF-315-14
Animal-Free Recombinant Murine SCF Peprotech Cat# AF-250-03
Poly (vinyl alcohol) Sigma-Aldrich Cat# P8136
miRCURY LNA RT Kit QIAGEN Cat# 339340
miRCURY LNA SYBR Green PCR Kit QIAGEN Cat# 339347
RD Western Diet Research Diets Cat# D12079B
OptiPrep density gradient medium Sigma-Aldrich Cat# D1556-250ML
Oil Red O Sigma-Aldrich Cat# O1391
Mayer’s Hematoxylin Thermo Fisher Scientific Cat# 72804
BLOXALL Blocking Solution Vector Laboratories Cat# SP-6000
ImmPACT NovaRED Peroxidase (HRP) Substrate Vector Laboratories Cat# SK-4805
D-Glucose solution Sigma-Aldrich Cat# G8769-100ML
Lipopolysaccharides from Escherichia coli O55:B5 Sigma-Aldrich Cat# L2880
Tissue-Tek O.C.T Compound Sakura FineTek Cat# 4583
Sucrose, 20% Sterile Solution VWR Cat# E543-100ML
10X Tris-EDTA, pH 7.4 Fisher Scientific Cat# BP24771
CountBright Absolute Counting Beads Thermo Fisher Scientific Cat# C36950
RNase A/T1 Mix Thermo Fisher Scientific Cat# EN0551
jetPRIME transfection reagent Polyplus Cat# 114-07
VECTASHIELD Antifade Mounting Medium with DAPI Vector Laboratories Cat# H-1200
Seahorse XF base medium Agilent Cat# 103335-100
Seahorse XF 100 mM pyruvate solution Agilent Cat# 103578–100
Novex 6% TBE Gels Thermo Fisher Cat# EC6265BOX
TruSeq SR Cluster Kit v3 - cBot - HS Illumina Cat# GD-401-3001
TruSeq SBS Kit v3 - HS Illumina Cat# FC-401-3002
T4 DNA Ligase NEB Cat# M0202
Trypsin-EDTA (0.05%) GIBCO Cat# 25300054
Opti-MEM GIBCO Cat# 31985–062
Glutamax GIBCO Cat# 35050061
Ficoll-Plaque Plus GE Healthcare Cat#17-1440-02

Critical Commercial Assays
miRNeasy Mini Kit QIAGEN Cat# 217004
Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225
Quant-iT RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat# R11490
Slide-A-Lyzer MINI Dialysis Device, 10K MWCO Thermo Fisher Scientific Cat# 88404
LS Columns Miltenyi Biotec Cat# 130-042-401
Subcloning Efficiency DH5a Competent Cells Thermo Fisher Scientific Cat# 18265017
Zyppy Plasmid Miniprep Zymo Research Cat# D4036
Seahorse XFe24 FluxPaks Agilent Cat# 102340-100
Seahorse XF Cell Mito Stress Test Kit Agilent Cat# 103015-100
BioAnalyzer High Sensitivity DNA Analysis Agilent Cat# 5067-4626
NEXTFLEX Small RNA Sequencing kit for Illumina Platforms Perkin Elmer Cat# NOVA-5132-06
Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714
Qubit Protein Assay Kit Thermo Fisher Scientific Cat# Q33211
PKH26 Red Fluorescent cell Linker kit Sigma Cat# PKH26GL-1KT
Exosome Spin Columns Invitrogen Cat# 448449
Seahorse XFe24 Cell Culture Microplates Agilent Cat# 100777-004
Vectastain Elite ABC Kit, Peroxidase (Rat IgG) Vector Laboratories Cat# PK-6104
nCounter® Low RNA Input Kit Nanostring Technologies Cat# LOW-RNA-48
Autoimmune Profiling Panel Nanostring Technologies Cat# XT-CSO-MAIPI1-12
V-Plex Mouse Custom Cytokine Kit MCP-1 Meso Scale Discovery Cat# K152A0H-1
MethoCult GF StemCell Technologies Cat#GF M3434

Deposited Data
miRNA-Seq This paper exRNA atlas: EXRNA-KJENS1DIAB, https://exrna-atlas.org/exat/datasets/EXR-KJENS1Lz9cll-AN
Experimental Models: Cell Lines
iBMDM-NFKB-Cas9 Cells Covarrubias et al., 2017 N/A

Experimental Models: Organisms/Strains
Mouse: B6.129P2-Apoetm1Unc/J Jackson Laboratories JAX:002052
Mouse: C57BL6/J Jackson Laboratories JAX:000664

Oligonucleotides
hsa-miR-146b-5p miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00204553
hsa-miR-16-5p miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00205702
hsa-miR-21-5p miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00204230
hsa-miR-99a-5p miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00204521
mmu-miR-378a-3p miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00204179
U6 snRNA (hsa, mmu) miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00203907
UniSp6 miRCURY LNA miRNA PCR Assay QIAGEN Cat# YP00203954
5′-IRD 800-labeled miR-146b IDT N/A
Primers for qRT-PCR and plasmid construction: see Table S1 This paper N/A

Recombinant DNA
psPAX2 Addgene RRID: Addgene_12260
pMD2.G Addgene RRID: Addgene_12259

Software and Algorithms
FlowJo FlowJo https://www.flowjo.com/
ImageJ NIH https://imagej.nih.gov/ij/
Photoshop CC Adobe https://www.adobe.com/products/photoshop.html
Prism 7 GraphPad https://www.graphpad.com/scientific-software/prism/
2100 Expert Bioanalyzer Agilent https://www.agilent.com/en/product/automated-electrophoresis/bioanalyzer-systems/bioanalyzer-software/2100-expert-software-228259
XFe Wave software Agilent https://www.agilent.com/en/products/cell-analysis/cell-analysis-software/data-analysis/wave-desktop-2–6
NTA 3.2 Malvern Panalytical https://www.malvernpanalytical.com/en/support/product-support/software/NanoSight-NTA-software-update-v3–2
NIS Elements BR 4.3 Nikon https://www.nikon.com/products/microscope-solutions/support/download/software/imgsfw/nis-br_v4300164.htm
nSolver Analysis Software Nanostring Technologies https://www.nanostring.com/products/analysis-software/nsolver