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. 2021 Apr 26;3(5):636–650. doi: 10.1038/s42255-021-00385-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, Immunoblot of WT and SLC25A33-Myc-Flag (A33-MycFlag) expressing HeLa cells treated with 40 µM ddC for 72 h (n = 1). b,c, Total and cytosolic mtDNA level in WT and SLC25A33–MycFlag (A33-MycFlag) expressing HeLa cells (n = 3 independent cultures (b) and n = 8 independent cultures (c)). d,e, ISG expression in WT and A33-MycFlag expressing HeLa cells treated with 40 µM ddC for 72 h analysed by RT–qPCR (d, n = 6 independent cultures) and RNA-Seq (e, n = 3 independent cultures). One hundred and eighty-one ISGs of 263 genes are upregulated (log₂(fold change) ≥1) in A33-MycFlag versus WT cells in the absence of ddC. The heatmap depicts the relative expression of 181 ISGs in ddC-treated A33-MycFlag versus ddC-treated WT cells. f, ISG expression in WT and A33-MycFlag expressing HeLa cells treated with indicated esiRNAs (n = 3 independent cultures). P values calculated using two-tailed unpaired t-test (b,c) or two-way ANOVA with Tukey’s multiple comparison test (d,f). Data are means ± s.e.m.

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