Skip to main content
. 2021 May 24;11:10753. doi: 10.1038/s41598-021-89673-8

Figure 1.

Figure 1

Iron supplementation reduces diet-induced weight gain. (a–g) Male C57BL/6J mice of 6 weeks of age were fed with an open

source control diet (Control; 10% kcal fat), a high-fat diet (HF; 60% kcal fat), or a high-fat diet supplemented with sodium ferrous citrate (HF + SFC) for 15 weeks. (a) Liver iron content. The values were corrected using tissue protein levels (n = 5–6). (b) Analysis of Hamp1 mRNA expression (n = 4–6). (c) Cumulative energy intake for 10–15 weeks per mouse per cage. (d) Body weight and food intake in the different groups (n = 11–12). Food intake was measured for 15 weeks. (e) Body weight gain from 0 to 15 weeks after administering (n = 11–12). (f, g) Longitudinal, (f) food, or (g) energy intake. Data are shown as mean ± SEM values. Statistical analysis was performed using one-way ANOVA followed by Holm–Sidak’s post hoc test. (a–e) *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant. (Significant differences vs. HF). (f, g) *p < 0.05. (Significant differences in HF vs. HF + SFC). #p < 0.05; ##p < 0.01; ###p < 0.001. (Significant differences in HF vs. Control).