Figure 3.

Iron supplementation reduces hepatic lipid accumulation. (a–d) Male C57BL/6J mice of 6 weeks of age were fed with each diet for 15 weeks as specified in Fig. 1. (a) Representative image of Hematoxylin–eosin (H&E) and Oil Red O stained liver sections. Scale bar is 50 μm. (b) Weight of liver (n = 5–6). (c, d) (c) Liver triglycerides (TG); and (d) total cholesterol (T-Cho) content (n = 5–6). The values were corrected by the tissue weight. Data are expressed as mean ± SEM values. Statistical analysis was performed using one-way ANOVA followed by Holm–Sidak’s post hoc test. **p < 0.01; ***p < 0.001; ns, not significant. (Significant differences vs. HF).