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. 2021 May 24;11:10736. doi: 10.1038/s41598-021-90271-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of CD13 leads to reduced bone volume and increased number of osteoclasts in 8–10 week old CD13-deficient mice. µCT reconstruction of cortical (a) and trabecular bone (b). (c–f) Bone morphometry by µCT analysis; (c) BV/TV(%); Bone volume/Tissue volume, (d) Trabecular number, (e) Trabecular Thickness, (f) Trabecular Separation. (g–i) Histomorphometric analysis of femurs of WT and CD13^KO. Histology of femurs with (g), BV/TV(%), (h) Oc.S/BS; % OCs per bone surface (i), histochemical detection of TRAP⁺ osteoclasts (purple, indicated by the arrow). (j) Mineral apposition rate (MAR), (k) Bone formation rate (BFR/BS) measured by Osteomeasure software (OsteoMetrics, Decatur, USA) (https://www.osteometrics.com). All samples were scanned, reconstructed, and analyzed in a Scanco µCT40 running Evaluation Program V6.6 (http://www.scanco.ch/en/systems-solutions/software.html). Histochemical analysis of TRAP⁺ osteoclasts in bone sections were imaged with Zeiss fluorescence inverted microscope and analyzed by using Zeiss Zen 2.0 Pro blue edition software (https://www.zeiss.com/content/dam/Microscopy/Downloads/Pdf/FAQs/zen2-blue-edition_installation-guide.pdf). Scale bars-×5; 200 µm, ×10; 100 µm, ×20; 50 µm. Data represents ± SD. (N = 6; WT, N = 7; CD13^KO. **p < 0.01).