Figure 3.

TRAP + multinucleated OCs are increased in the absence of CD13 in vitro. (a) Primary murine BM- derived osteoclast progenitor cells grown on bovine cortical slices in presence of M-CSF and RANKL led to increased OCs size and number of nuclei/OC in CD13^KO cells compared to WT at d5. (b) Average # of cells with > 3 nuclei/field and (c) Average cell area per OCs in CD13^KO are significantly larger than the WT cells. (d) Area of resorption is significantly higher in CD13^KO than WT OCs grown on osteoplates for d10 by phase contrast imaging. Osteoclasts on cortical slices and cluster of pits formed were imaged using a light microscope (Olympus Scientific), using Olympus cellSens Dimension V0118 software (Olympus Scientific) (https://www.olympus-lifescience.com/en/software/cellsens/) and the area of resorption was quantified by Image J (https://imagej.nih.gov/ij/) (e) and total number of nuclei/well of both genotypes (f) are shown. Scale bars-(a) 50 µm; (d) 200 µm. Data represents ± SEM of three independent experiments. N = 6/genotype, **p < 0.01, *p < 0.05.