Figure 4.

Treatment with CD13 blocking Ab leads to increased multinucleated OC formation in WT cells. (a). TRAP staining of WT, WT + SL13 and CD13^KO BM-derived osteoclast progenitor cells grown in presence of M-CSF and RANKL after 5d on plastic are shown in “red” color. (b). Quantification of Trap⁺ OC with > 3 nuclei/cell. TRAP⁺ osteoclasts were imaged with Zeiss fluorescence inverted microscope and analyzed by using Zeiss Zen 2.0 Pro blue edition software (https://www.zeiss.com/content/dam/Microscopy/Downloads/Pdf/FAQs/zen2-blue-edition_installation-guide.pdf). Scale bar; 200 µm. Data represents ± SEM of three independent experiments. N = 3/genotype, **p < 0.01. Magnification ×5.