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. 2021 May 24;12(6):532. doi: 10.1038/s41419-021-03814-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A The mRNA expression of PEPT1 in SW480 and SW620 cells after HDAC inhibitors treatment. Cells were treated with DMSO, 1 μM SAHA for 48 h or 0.5 μM TSA for 24 h. Data are shown as means ± SD, n = 3, two-tailed unpaired t-test, ns, no significance, **P < 0.01, ***P < 0.001, ****P < 0.0001. B Immunoblotting confirmed HDAC inhibitors treatment in SW480 and SW620 cells. C–D Knockdown of HDAC1 activated PEPT1 mRNA and protein expression in SW480 and SW620 cells, respectively. SiNC, cells transfected with negative control siRNA, siHDAC1#1, siHDAC1#2, two siRNAs for HDAC1. Data are shown as means ± SD, n = 3, two-tailed unpaired t-test, *P < 0.05, **P < 0.01, ****P < 0.0001. E ChIP-qPCR analyses of H3K18/H3K27Ac occupancy at PEPT1 promoter in SW480 and SW620 cells after SAHA (1 μM for 48 h) treatment. Data are shown as means ± SD, two-tailed unpaired t-test, **P < 0.01, ***P < 0.001, ****P < 0.0001. F PEPT1 mRNA expression in CRC47, 50, 51, 53, 55. Data are shown as means ± SD, two-tailed paired t-test, **P < 0.01, ***P < 0.001. G ChIP-qPCR analyses of H3K18/K27Ac occupancy at the PEPT1 promoter in five paired CRC tissues. Data are shown as means ± SD, two-tailed unpaired t-test, ***P < 0.001, ****P < 0.0001.