Table 1.
Primer sequences for gene amplification
| Gene | Primer sequence (5′-3′) | Product size (bp) | Accession No | |
|---|---|---|---|---|
| 18S rRNA |
Forward: Reverse: |
AAACGGCTACCACATCCAAG TTGCCCTCCAATGGATCCT | 159 | NR_046237 |
| ACC |
Forward: Reverse: |
CAACCACTACGGCATGACTCA CGCAGAAGCAGCCCATTACTT |
155 | NM_022193 |
| FAS |
Forward: Reverse: |
TGCTCCCAGCTGCAG GCCCGGTAGCTCTGGGTGTA |
107 | NM_017332 |
| Acox1 |
Forward: Reverse: |
CAAGGAGAGTGCTACGGGTTA TTCAGGTAGCCGTTATCCAT |
137 | NM_017340 |
| SREBP-1 |
Forward: Reverse: |
GCAAGGCCATCGACTACATC TTTCATGCCCTCCATAGACAC |
161 | XM_213329 |
| Insig 1 |
Forward: Reverse: |
TTGTCGGCTTATTGTATCCCT GCACATTATTGGCGAAATCT |
147 | NM_022392 |
| Insig 2 |
Forward: Reverse: |
GGCGGAAGGAGAGACGGAGTC AAGCCAGGAACACGCCAATGA |
128 | NM_178091 |
| Acly |
Forward: Reverse: |
GCAGACCAGAAGGGCGTGAC CACACTGCCTGGGCGATACAG |
135 | NM_016987 |
| Fads1 |
Forward: Reverse: |
GTTTGTGTGGGTGACGCAGAT TTGAAGGCTGACTGGTGAACG |
117 | NM_053445 |
| Gpam |
Forward: Reverse: |
CCT GTG GGC ATC TCG TAT GAT TTC CGC AGC ATT CTG ATA AC |
122 | NM_017274 |
| Dgat1 |
Forward: Reverse: |
CAGATGGGGCTGCTGCTACAT GGCGGCACCACAGATTGACAT |
175 | NM_053437 |
| GS2 |
Forward: Reverse: |
GACACTGAGCAGGGCTTTTCC GAGGAGGGCCTGGGATACTT |
90 | NM_013089 |
| GYG1 |
Forward: Reverse: |
TCGCCAGCCCACAGGTT CACCACTGTCCAAGACATCTACCA |
90 | NM_031043.2 |
| PEPCK |
Forward: Reverse: |
GAAAGTTGAATGTGTGGGTGAT TTCTGGGTTGATGGCCCTTA |
79 | NM_198780 |
| G6PC1 |
Forward: Revers: |
GTATGGATTCCGGTGCTT AATGCCTGACAAGACTCCA |
132 | NM_013098 |