Table 1.

The regression analysis of variance for Plackett–Burman design model for E. coli W3110

Source	Factors	Low level ( − 1)	High level ( − 1)	Sum of Squares	Mean Square	F Value	P value
	Model			13.331	1.333	277.721	0.0467*
A	glucose	30.00	50.00	10.01	10.01	2085.444	0.0139***
B	(NH4)2SO4	7.50	12.50	1.658	1.658	345.340	0.0342**
C	Yeast extract	1.00	3.00	0.156	0.156	15.365	0.2751
D	KH2PO4	0.60	1.00	0.087	0.087	18.063	0.4471
E	MgSO4	0.30	0.70	0.002	0.002	0.340	0.1458
F	Salt solution	0.75	1.25	0.006	0.006	1.174	0.4745
G	Temperature	30.00	37.00	0.002	0.002	0.340	0.1438
H	CaCO3	0.30	0.40	0.626	0.626	130.340	0.0756
J	Inoculum	100.00	200.00	0.023	0.023	4.694	0.0553
K	β-alanine	1.00	3.00	0.897	0.897	186.778	0.0465*
L	IPTG	1.00	3.00	0.023	0.023	4.694	0.5153

The regression analysis of variance for Plackett–Burman design model for E. coli W3110

Values of “Prob. > F” less than 0.0300, 0.0400, and 0.0500 indicate that model terms are extremely significant (***), highly significant (**), and significant (*), respectively