Fig. 5. Migration pattern of eneteroendocrine lineages.

a Spatio-temporal analysis of enteroendocrine cells. Central plot: Scatter plot of the center of mass (COM) in our spatial zonation reconstruction vs. temporal COM based on single cell time stamps form Gehardt et al.¹² (“Methods”). Dots colored by ‒log10(spatial zonation q-value). Peripheral plots show reconstructed temporal (red) and spatial (blue) profiles for early crypt-confined genes (green fonts, bottom row), intermediate-late crypt/villus bottom-confined genes, expressed in cells that might be migrating more slowly (cyan fonts, top row) and late villus-localized genes, expressed in cells that might be migrating more rapidly (black fonts, right column). Light areas in plots denote the SEM. b Representative smFISH images of the enteroendocrine crypt-zonated gene Sst (red). Insets are blow-ups of the Sst+ cells at the crypt and bottom villus. Ada in gray marks the villi tips. Scale bars, 50 μm in the large image and 10 μm in the insets. c Quantification of Sst+ enteroendocrine D-cells in crypt and villus bottom, middle and tip over 3 mice. Fisher exact test (two-sided) for the frequencies of D-cells between the two lower zones and two upper zones p = 3.8 × 10⁻⁵. Data are presented as mean values ± SD. Source data are provided as a Source data file.