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. 2021 May 24;11:10740. doi: 10.1038/s41598-021-89938-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bisbee detects protein expression confirmed splice events with high sensitivity. (A) The number of events with protein expression evidence of differential splicing is plotted against the total number of events passing the threshold for four different differential splicing methods: beta binomial (bbd—black), SplAdder’s test (sp—red), t-test on all PSI (tt—blue), t-test on PSI with depth > 10 (tt-d10—cyan). (b) The number of mass spectrometry confirmed outlier events is plotted against the total number of events passing the threshold for five different methods: bisbee outlier (black), median absolute deviation (red), median absolute deviation with depth > 10 (magenta), interquartile range (blue), and interquartile range with depth > 10 (cyan).