Table 1.
Antigens/Epitopes | References | Main characteristics | Analytical methods | ||
---|---|---|---|---|---|
Synthetic | Multianalysis profiling | Surface plasmon resonance | Western Blotting/ELISA*** | ||
Biotin Sulfone Tagged Oligomannosides (BSTOs), schematic representation of the epitope | |||||
Manα-1,2Man | X | X | X | ||
Manα-1,2Manα-1,2Manα-1,2Man | X | ||||
Manβ-1,2Man | X | ||||
Manβ-1,2Manβ-1,2Man | Mat & Meths | Synthesized for the present study | X | ||
Manβ-1,2Manβ-1,2Manβ-1,2Man | X | X | X | ||
Native | |||||
Phosphopeptidomannan (mannan) From C. albicans VW32 (Serotype A) |
27, 31–33, 55 | Complex cell wall surface polymer. Structure determined by NMR and sequential depolymerization of constitutive oligomannosides used for diagnostic purposes | *** | ||
Phospholipomannan | 18, 56, 57 |
Glycoplipid of the mannoinositolphospho ceramide family Present at cell wall surface Shed in contact with host cells and has immunomodulatory properties |
X | ||
From C. albicans VW 32 (serotype A) | 19 | Polysaccharide moiety consisting of β-mannosides up to 14 residues | X | ||
From C. albicans NIH-B (serotype B) | 20 | Polysaccharide moiety consisting of β-1,2 mannotriose | X | ||
From C. albicans BPW17 (serotype A) | 58 | PLM identical to that of VW32. Used for genetic manipulations | X | ||
From C. albicans BPW17 depleted in bmt6 | 21 | Polysaccharide moiety truncated with a β-mannotriose | X |