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. 2021 May 24;12:3059. doi: 10.1038/s41467-021-23285-8

Fig. 3. AAV8-mediated reintroduction of PPDPF rescues the phenotype of PPDPF-null in PPDPF-LKO mice fed a chow diet for 8 months.

Fig. 3

The body weight (a), liver weight (b), TG test (c) and NEFA test (d) of WT + AAV8-con (n = 8), LKO + AAV8-con (n = 7), and LKO + AAV8-PPDPF (n = 7) mice at 8 months on chow diets. Mean ± SEM, (a) **p = 0.0017 (WT + AAV8-con Vs LKO + AAV8-con), ****p < 0.0001 (LKO + AAV8-con Vs LKO + AAV8-PPDPF), (b) **p = 0.0055 (WT + AAV8-con Vs LKO + AAV8-con), **p = 0.01 (LKO + AAV8-con Vs LKO + AAV8-PPDPF), (c) ****p < 0.0001 (WT + AAV8-con Vs LKO + AAV8-con), ****p < 0.0001 (LKO + AAV8-con Vs LKO + AAV8-PPDPF), (d) ****p < 0.0001 (WT + AAV8-con Vs LKO + AAV8-con), ****p < 0.0001 (LKO + AAV8-con Vs LKO + AAV8-PPDPF) by two-tailed unpaired Student’s t test. e Representative images of H&E and Oil Red O staining of liver sections from the mice injected with indicated adenovirus at 8 months. Scale bars, 100 um. f The mRNA expression levels of lipogenesis-related genes in WT + AAV8-con (n = 6), LKO + AAV8-con (n = 6) and LKO + AAV8-PPDPF (n = 6) mice at 8months. Mean ± SEM, SREBP1: **p = 0.0015, *p = 0.0463; FASN: **p = 0.0050, *p = 0.0029; ACLY: *p = 0.0322, *p = 0.0123; PPARG: *p = 0.0134, *p = 0.0281; ME: *p = 0.459, *p = 0.011 by two-tailed unpaired Student’s t test. All experiments were repeated three times independently.