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. 2021 May 24;12:3059. doi: 10.1038/s41467-021-23285-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Co-immunoprecipitation assay in 293T cells co-transfected with Flag-Raptor and HA-PPDPF. c, d Examination of the interaction between endogenous PPDPF and Raptor by co-immunoprecipitation. e PPDPF interacts with Raptor in vitro, purified GST was used as a control. f GST-pull down assay using different truncation mutants of PPDPF. g, h 293 T cells were transfected with control vector and PPDPF WT, PPDPF mut, HA-Raptor plasmid, respectively. After 48 h, co-immunoprecipitation assay was performed. i GST-pull down assay examining the interaction between GST-PPDPF, GST-PPDPF mut with Raptor. j The ubiquitination of Raptor in 293T cells was examined using immunoprecipitation with Raptor antibody. All experiments were repeated 3 times independently.