Fig. 2.

KDM4B associates with CCAR1. a Schematic diagram of the method used to purify the KDM4B-interacting complex from FLAG-KDM4B-expressing cells. Lane 1, control preparation from the nuclear extract of normal HeLa cells; lane 2, KDM4B-interacting complex from the nuclear extract of KDM4B-expressing HeLa cells. b KDM4B-interacting complexes. c TRAP staining of BMMs expressing either control shRNA or Ccar11 shRNA1. Scale bar, 75 μm. d, e CCAR1 expression during osteoclast differentiation. qRT-PCR (d) and immunoblot (e) results. f Coimmunoprecipitation of FLAG-KDM4B and HA-CCAR1. g Endogenous interaction of KDM4B and CCAR1 in BMMs. h, i Mapping the binding domain of KDM4B and CCAR1. JmjN N-terminal jumonji domain, JmjC jumonji C-terminal domain, PHD plant homeodomain finger domain, Tudor Tudor domain. SAP, SAF-A/B, Acinus, and PIAS DNA/RNA binding motif. The data are presented as the mean ± SD values of three independent experiments [one-way ANOVA in (c, d)]. *P < 0.05; **P < 0.01; ***P < 0.001. See also Supplementary Fig. 2