Fig. 4.

The KDM4B–CCAR1–MED1 axis is required for osteoclastogenesis. a, b MED1 expression during osteoclast differentiation. qRT-PCR (a) and immunoblot (b) results. c TRAP staining of BMMs expressing either control shRNA or Med1 shRNA. Scale bar, 75 μm. d Heatmap of normalized tag densities on KDM4B, CCAR1, and MED1 peaks (2 kb up/downstream of the peak centers) (left panels). Quantifications of MED1 tag counts are shown in the box plots (right panel). The boxes enclose the 25th to 75th percentile values. The whiskers extend to the 10th and 90th percentiles. The central horizontal bar indicates the median. ****P < 0.000 1 (Kolmogorov–Smirnov test). e Representative UCSC Genome Browser tracks showing co-occupancy of KDM4B, CCAR1, and MED1 at Fosl2 and Tpm1. f mRNA expression levels of Fosl2 and Tpm1 in cells expressing control shRNA or Med1 shRNA. The data are presented as the mean ± SD values of three independent experiments [one-way ANOVA in (a, c)]. *P < 0.05; **P < 0.01; ***P < 0.001. See also Supplementary Fig. 4