Skip to main content
. 2021 May 24;11:10765. doi: 10.1038/s41598-021-90143-4

Figure 2.

Figure 2

The effect of CYP inhibitors on SLCO1B3 expression. 22Rv1 cells were treated with four CYP17 inhibitors, ketoconazole (Keto), abiraterone (ABI), galeterone (GAL), or seviteronel (SEV), in media supplemented with 1 nM testosterone for 24 h. Cells were treated with ketoconazole and abiraterone at a concentration of 20 µM and galeterone and seviteronel at a dose of 10 µM based on precedents set for in vitro experiments in previous publications. RNA was extracted and assessed for changes in SLCO1B3 expression using qPCR. Gene expression was normalized to expression of β-actin. All CYP17 inhibitors but seviteronel produced statistically significant induction of SLCO1B3 in 22Rv1 cells. Statistical tests were performed against CRTL + T. Data represent three independent experiments with triplicate measurements. **P < 0.001, ***P < 0.0001, ****P < 0.00001.