Table 7.
Strategies suggestion to perform a study on AM or AM derived products in the field of bone regeneration.
AM use strategy | Preparation method | Disposition | Use | Study design | Expected results |
---|---|---|---|---|---|
AM as a membrane for GBR procedure | Lyophilization or Decellularization + Lyophilization of AM | Over the defectOne layer | Alone or associated with a bone substitute | 1. Defect 2. Defect + AM 3. Defect + bone substitute 4. Defect + bone substitute covered by AM 5. Defect + bone substitute covered by conventional membrane use for GBR |
- Orthotopic bone regeneration supported by quantitative analysis of 3D-Radiography and histological analysis - Angiogenic and anti-fibrotic effect of AM and its derivatives supported by histological and/or immunohistochemical analysis - Statistical significance |
AM as a scaffold for bone tissue engineering construct | Decellularization of AM | Inside or over the defect | Seeded with human mesenchymal stromal cells | 1- Defect 2- Defect + cells 3- Defect + AM 4- Defect + AM-seeded cells |
|
AM cells-based strategies (AECs or AMSCs) for bone tissue engineering construct | AM-cells cultured in basal or osteogenic medium | Inside the defect | Seeded on a bone substitute | 1- Defect 2- Defect + AM cells 3- Defect + bone substitute 4- Defect + AM cells seeded on the bone substitute 5- Defect + hADSCs or hBMSCs seeded on the bone substitute |
hADSCs, human adipose stromal cells; hBMSCs, human bone marrow stromal cells; GBR, guided bone regeneration.