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. 2021 May 11;9:661332. doi: 10.3389/fbioe.2021.661332

Table 7.

Strategies suggestion to perform a study on AM or AM derived products in the field of bone regeneration.

AM use strategy Preparation method Disposition Use Study design Expected results
AM as a membrane for GBR procedure Lyophilization or Decellularization + Lyophilization of AM Over the defectOne layer Alone or associated with a bone substitute 1. Defect
2. Defect + AM
3. Defect + bone substitute
4. Defect + bone substitute covered by AM
5. Defect + bone substitute covered by conventional membrane use for GBR
- Orthotopic bone regeneration supported by quantitative analysis of 3D-Radiography and histological analysis
- Angiogenic and anti-fibrotic effect of AM and its derivatives supported by histological and/or immunohistochemical analysis
- Statistical significance
AM as a scaffold for bone tissue engineering construct Decellularization of AM Inside or over the defect Seeded with human mesenchymal stromal cells 1- Defect
2- Defect + cells
3- Defect + AM
4- Defect + AM-seeded cells
AM cells-based strategies (AECs or AMSCs) for bone tissue engineering construct AM-cells cultured in basal or osteogenic medium Inside the defect Seeded on a bone substitute 1- Defect
2- Defect + AM cells
3- Defect + bone substitute
4- Defect + AM cells seeded on the bone substitute
5- Defect + hADSCs or hBMSCs seeded on the bone substitute

hADSCs, human adipose stromal cells; hBMSCs, human bone marrow stromal cells; GBR, guided bone regeneration.