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. 2021 May 11;8:630107. doi: 10.3389/fmolb.2021.630107

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Copyright © 2021 Mould, Botchway, Parkinson, Thomas, Guy, Bell and Nunn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro effects of CBD on mitochondrial ROS and mitochondrial Ca²⁺ production in MCF7 cells. (A) Effects of cannabidiol (CBD) on normalized MitoSOX intensity as a marker of mitochondrial reactive oxygen species (ROS) production. (B) Effects of CBD on normalized Rhod2 intensity as a marker of mitochondrial Ca²⁺ production. Box shows median with 25th and 75th percentiles, and whiskers indicate minimum and maximum values. n = 8/group; ** = p < 0.01, *** = p< 0.001; data were analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons using GraphPad Prism (United States).