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. 2021 May 11;12:670992. doi: 10.3389/fimmu.2021.670992

Figure 6.

Figure 6

Cytokine profiles in the supernatants of lymphoproliferation with the peptides (IFN-γ, IL-2, IL-4, IL6, IL17A and TNF-α cytokines represented in A–F respectively). The supernatants obtained from the lymphoproliferation after 72 h of incubation with the peptides were analyzed for cytokine levels by CBA. The supernatant from the lymphocytes cultivated in the absence of any kind of stimulus (Lymphocytes) was used as a negative control and the supernatant from the lymphocytes cultivated with Concanavalin A was used as a positive control (ConA). The data shown are from two independent experiments. Data were analyzed by one-way ANOVA followed by Tukey’s post-test and confirmed by “t” test, where *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 in comparison to supernatant from the lymphocytes without stimulus (Lymphocytes).