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. 2021 May 11;12:654714. doi: 10.3389/fphar.2021.654714

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Copyright © 2021 Xia, Liu, Yang, Liu, Luo, Yang, Xie, Zeng, Xu, Ling, Zeng, Xu, Fang, Wang, Tong, Jin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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BYD activated Wnt/β-catenin signaling pathway to the promoted osteoblastic formation in vitro via ESR1. (A) Relative proliferation of various concentration of BYD (50, 100, 200, 400 μg/ml). (B) Alkaline phosphatase (ALP) activity. (C) Result of Alizarin Red staining. (D) β-catenin localization analysis result. (E) Representative images of alizarin red staining and immunofluorescence staining of β-catenin. (F) Representative images of western blots of RUNX2, β-catenin, cylindD1, and ESR1. Quantitative analysis results of protein expressions analysis of (G) RUNX2, (H) β-catenin, (I) CyclinD1, and (J) ESR1. Quantitative analysis results of mRNA expression analysis of (K) RUNX2, (L) β-catenin, (M) CyclinD1, and (N) ESR1. THE exact p-value can be found in the corresponding histogram.