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. 2021 May 24;11:10734. doi: 10.1038/s41598-021-90220-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The construction of ABI1-TSV-11-overexpressing LoVo and SW480 cell models in vitro. (a) Relative expression levels of endogenous ABI1-TSV-11 in the normal colorectal cell line (CRL-1541) and CRC cell lines (LoVo, SW480, SW620) (RNA sequencing). (b) and (c) fluorescence images and quantitative real-time PCR analysis using ABI1 universal primers of in LoVo-ABI1-TSV-11 and SW480-ABI1-TSV-11 cell lines after lentivirus infection and puromycin selection, as well as their controls. (d) Western blotting analysis of ABI1-TSV-11 in LoVo-ABI1-TSV-11 and SW480-ABI1-TSV-11 cell lines and their corresponding controls using HA antibody.