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. 2021 May 24;11:10782. doi: 10.1038/s41598-021-90324-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt7b overexpression reduces cell proliferation in trabecular bone region. (A) Representative images of H&E-stained sections of the distal femur at P33. (B) Representative images of the distal femur labeled with EdU at P33. EdU signal is in green and DAPI nuclei staining in blue. (C) EdU labeling index at the chondro-osseous junction of distal femur (100 µm region immediately below the growth plate). *: P < 0.05, two-way ANOVA, n = 3 (females). GP: growth plate.