. 2021 May 25;11(31):18832–18839. doi: 10.1039/d0ra09874d

A ₄₅₀ readings from TMB substrate and stop solution ± streptavidin-HRP (S-HRP) for (a) non-biotinylated and biotinylated miR-21 biosensors and (b) non-biotinylated miR-21 or miR-223 biosensors hybridised with 10⁻⁷ M 5′-bio-miR-21.

Spectrophotometric analysis at 450 nm (A₄₅₀)
(a) Non-biotinylated or biotinylated (bio) miR-21 biosensors			(b) Non-biotinylated miR-21/miR-223 biosensors + 5′-bio-miR-21
Comp-miR-21 − S-HRP	Comp-miR-21 + S-HRP	3′-Bio-comp-miR-21 + S-HRP	Comp-miR-21 − S-HRP	Comp-miR-223 + S-HRP	Comp-miR-21 + S-HRP
0.000^a	0.010 ± 0.002	0.123 ± 0.014	0.000^a	0.008 ± 0.003	0.091 ± 0.006

Baseline response − streptavidin has been subtracted from the data, values are expressed as mean ± SD (n = 3). Oligonucleotides' details are provided elsewhere (ESI, Fig. S1).

A 450 readings from TMB substrate and stop solution ± streptavidin-HRP (S-HRP) for (a) non-biotinylated and biotinylated miR-21 biosensors and (b) non-biotinylated miR-21 or miR-223 biosensors hybridised with 10−7 M 5′-bio-miR-21.

A ₄₅₀ readings from TMB substrate and stop solution ± streptavidin-HRP (S-HRP) for (a) non-biotinylated and biotinylated miR-21 biosensors and (b) non-biotinylated miR-21 or miR-223 biosensors hybridised with 10⁻⁷ M 5′-bio-miR-21.