Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 20;46(1):134. doi: 10.3892/or.2021.8085

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

PMC Copyright notice

Figure 2. — PCED1B-AS1 knockdown represses PDAC cell proliferation, invasion, and EMT. (A) RT-qPCR was utilized to investigate the expression of PCED1B-AS1 in CFPAC-1 and SW1990 cells transfected with si-NC or si-PCED1B-AS1. (B and C) CCK-8 and EdU staining assay were used to assess the effect of PCED1B-AS1 knockdown on proliferation of CFPAC-1 and SW1990 cells. (D) Transwell invasion assays were used to assess the effects of PCED1B-AS1 knockdown on the invasion of CFPAC-1 and SW1990 cells. (E) Western blotting was used to assess the expression of the EMT markers, E-cadherin, N-cadherin, Vimentin and Snail, following transfection. *P<0.05, **P<0.01, ***P<0.001 vs. si-NC. siRNA, small interfering RNA; si-NC, si-negative control; PDAC, pancreatic ductal adenocarcinoma; PCED1B-AS1, PC-esterase domain containing 1B-antisense RNA 1; RT-qPCR, reverse transcription-quantitative PCR; EMT, epithelial-mesenchymal transition; CCK-8, Cell Counting Kit-8.