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. 2021 May 20;46(1):136. doi: 10.3892/or.2021.8087

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Effects of ND-09 on JAK/STAT signaling protein levels. (A) Protein levels of JAK2, p-JAK2, JAK3, and p-JAK3 in K562 cells treated with ND-09 were evaluated by western blot analysis. (B) Protein levels of STAT3, p-STAT3, STAT5, and p-STAT5 in K562 cells treated with ND-09 were evaluated by western blot analysis. Results were quantified by densitometry analysis of the bands and were normalized to GAPDH (internal control). Samples were derived from the same experiment, and blots were processed in parallel. Values represent the average of three independent experiments. Data are presented as mean ± SEM (n=3). *P<0.05, **P<0.01 compared to the untreated control group.