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. 2021 May 20;46(1):136. doi: 10.3892/or.2021.8087

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effects of ND-09 on PI3K/AKT signaling protein levels. (A) Protein levels of PTEN, PI3K-p110α, PI3K-p110β, PI3K-p110γ, p-PI3K p85/p55, and PI3K-p85 in K562 cells treated with ND-09 were evaluated by western blot analysis. (B) Protein levels of AKT, p-AKT, mTOR, and p-mTOR in K562 cells treated with ND-09 were evaluated by western blot analysis. Results were quantified by densitometric analysis of the bands and were normalized to GAPDH (internal control). Samples were derived from the same experiment, and blots were processed in parallel. Values represent the average of three independent experiments. Data are presented as mean ± SEM (n=3). *P<0.05, **P<0.01 compared to the untreated control group.