Figure S5.

Glycan modifications of anti-ERV-K102 IgG and NETosis in SLE neutrophils. (A) Glycan modifications on anti-ERV-K102 IgG (healthy and SLE) detected using biotinylated lectins and quantified by a Luminex assay. Lectin binding was quantified for untreated IgG (left panel) and PNGase F–treated IgG (right panel). Relative units were calculated by normalizing MFI of each lectin signal by MFI of total human IgG for each sample. Mann–Whitney t test was performed to calculate statistical significance. *, P < 0.05; **, P < 0.01. (B) Representative microscopy images of neutrophils from SLE patients stimulated with ERV-K102 immune complexes (ICs) generated with healthy plasma (n = 5) or SLE plasma (n = 5). (C) NETs were quantified by measuring the area of Hoechst staining per cell in ImageJ. For each condition per donor plasma, four images were recorded, four cells were measured per image, and the average area per image was plotted. Scale bars, 75 µm. RU, relative unit.