Figure 3.

Effect of HTT priming in FD-stimulated HaCaT keratinocyte preconditioned media on MAPK derived NF-κB inflammatory signaling in integrated HDFs. Levels of molecular mediators were assessed by western blot analysis for (A) MAPK and (B) NF-κB. (C) Evaluation of NF-κB p65 nuclear translocation by immunofluorescence analysis. Evaluations were carried out in triplicates (n = 3) and indicated as means ± SD. “*” and “**” respectively denote p < 0.05 and p < 0.01 if they are significantly different from those of HPM-FD treated HDFs “#”.