Figure 5.

Ilimaquinone increases ROS generation in MCF-7 cells. (A) Cells were treated with DMSO or ilimaquinone at 1, 3, and 5 h. (B) Histogram of ROS production of ilimaquinone at the indicated time interval in MCF-7 cells. Columns represent means; bars represent S.D. * p < 0.05, ** p < 0.01 compared with the control group. (C) Statistical analysis of ROS generation after the treatment of ilimaquinone alone or in the combination of 500 μM glutathione (GSH) or 300 μM H₂O₂ alone for 3 h (n = 5). Data are represented as means ± S.D. ** p < 0.01 compared with the control group. # denotes p < 0.05 when compared with ilimaquinone (10 μM) alone group. ## denotes p < 0.05 when compared with ilimaquinone (20 μM) alone group.