Figure 2.

Total pancreatectomy with islet auto-transplantation (TPIAT): (a) Resected pancreas after removal of duodenum and vessels; (b) Enzymatic and mechanical digestion of the resected pancreas; (c) After collection of digested pancreatic tissue, samples are taken for microscopic inspection to assess islet size, number, and morphology with dithizone staining. White arrows indicate stained islet cells; (d) Digested pancreatic tissue with islets were transferred to an infusion bag for auto-transplantation.