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Effects of zinc sulfate on osteogenesis-related phenotypes in co-cultures of hPDCs and THP-1 cells. (A) Representative image of co-cultured hPDCs and THP-1 cells after 10 days of culture. (B) Effects of zinc sulfate treatment on the expression and bioactivity of ALP, mineralization, and calcium levels in co-cultures of hPDCs and THP-1 cells. Black arrow, osteoblasts; red arrow, multinucleated osteoclasts. ** p < 0.01. *** p < 0.001.
