Figure 3.

Ae. aegypti, Ae. Albopictus, and Cx. quinquefasciatus mosquitoes are resistant to OROV infection after feeding on infected mice. (A) Scheme of the experimental design using OROV viremic mice to orally infect mosquitoes. Four- to five-week-old AG129 mice were inoculated with 10⁵ p.f.u. of OROV by intraperitoneal (IP) injection. After three days, mice were anaesthetized and then mosquitoes (5- to 7-day-old females) were allowed to take blood meals in the OROV-infected mice. Seven and 14 days after the blood meal, mosquitoes were collected and tested individually for the presence of OROV. (B) Seven d.p.f OROV RNA levels of mosquito abdomen that fed on OROV-infected mice. RNA levels were quantified by RT-qPCR. Two strains of Aedes aegypti were tested, the BH strain (wild-caught population from Belo Horizonte, Brazil) and the BKK strain (laboratory Bangkok strain). One strain of Aedes albopictus was tested, the BH strain (wild-caught population from Belo Horizonte, Brazil). One strain of Culex quinquefasciatus was tested, the BH strain (wild-caught population from Belo Horizonte, Brazil). (C) Fourteen d.p.f OROV RNA levels of mosquito abdomen that were fed on OROV-infected mice.