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. 2021 Apr 26;10(5):1020. doi: 10.3390/cells10051020

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CD276-CAR NK-92 cells as well as parental NK-92 cells were co-incubated with calcein-labeled melanoma cell lines at indicated E:T ratios for 2 h. Specific cytotoxicity was assessed using a calcein release assay and is shown as mean ± SD, n = 3. Prior to the experiments, NK-92 and CD276-CAR NK-92 cells were either cultivated under hypoxic conditions (5% CO₂, 1% O₂) for 24 and 168 h (a–c), pre-treated with indicated concentrations of TGFβ (d–f), sodium l-lactate (g–i) or supernatants, previously collected from human fibroblast and cancer-associated fibroblast (CAF) culture (j–l) as well as melanoma cell line culture (m–o), for 48 h; **** p < 0.0001.

CD276-CAR NK-92 cells as well as parental NK-92 cells were co-incubated with calcein-labeled melanoma cell lines at indicated E:T ratios for 2 h. Specific cytotoxicity was assessed using a calcein release assay and is shown as mean ± SD, n = 3. Prior to the experiments, NK-92 and CD276-CAR NK-92 cells were either cultivated under hypoxic conditions (5% CO₂, 1% O₂) for 24 and 168 h (a–c), pre-treated with indicated concentrations of TGFβ (d–f), sodium l-lactate (g–i) or supernatants, previously collected from human fibroblast and cancer-associated fibroblast (CAF) culture (j–l) as well as melanoma cell line culture (m–o), for 48 h; **** p < 0.0001.