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. 2021 Apr 26;10(5):1020. doi: 10.3390/cells10051020

Figure 4.

Figure 4

GFP-transduced cell lines FM-3, Mel-Juso and WM115 were grown as three-dimensional melanoma spheroids for 72 h before being co-incubated with CD276-CAR NK-92 or parental NK-92 cells for 96 h. Integrated fluorescence intensity of melanoma spheroids (FM-3, Mel-Juso and WM115) was measured regularly using the Celigo S imaging cytometer. Representative fluorescence images of WM115 spheroids from indicated time points (a) as well as all melanoma spheroids after 96 h (b) are shown. Integrated fluorescence intensity was compared to untreated control spheroids and is shown as mean ± SD, n = 3 (c–e).